Endophytic actinomycetes have been known as a promising source of new antibiotics against susceptible and resistant forms of microorganisms. In this study, we isolated, identified endophytic actinomycetes that isolated from Neesia altissima based on phylogenetic analysis of DNA sequence. The antibacterial bioactive compounds were also isolated from the endophytic
actinomycetes based on elucidation of the structure. The endophytic actinomycetes isolated from roots, barks and fresh leaves of Neesia altissima collected from Halimun-Salak Mount were screened for their potential against pathogenic bacteria using crude extract dilution and diffusion disc methods and then identified. The crude extracts obtained from two endophytic
actinomycetes that exhibited potential antibacterial activity by showing clear zone surrounding the pathogenic bacteria. Phylogenetic tree was constructed using a nearly complete sequence within the 16S rRNA gene. Isolation and identification of bioactive compounds were carried out using TLC, NMR and MS analyses. Identification of the potential endophytic actinomycetes based on phylogenetic analysis of DNA sequence generated from 16S rRNA region determined as Streptomyces sp. UICC B-92. Crude extract of Streptomyces sp. UICC B-92 showed antagonistic activity against Gram positive bacteria, Bacillus cereus ATCC 10876 and Staphylococcus aureus ATCC 25923. The bioactive compound that isolated from endophytic
actinomycetes was phenazine derivative. The novel bioactive compound of phenazine derivative would be expedient to modify the same in view of another implication, such as anticancer, antifungal and antioxidant.