Actinomycetes are Gram positive bacteria that like molds morphologically. Both of them have similarly for thegrowing pattern, but each of them have specifically medium for the growth and the storage. The aim of this research is to study the appropriate medium that can use for growing and storaging endophytic actinomycetes especially strain UICC B-92. Endophytic actinomycetes strain UICC B-92 is actinomycetes that isolated from the plant tissue of Neesia altissima Bl. The result showed International Streptomyces Project number 2 (ISP2) medium that use for isolating endophytic actinomycetes is more effective than Starch Casein Agar (SCA) and International Streptomyces Project nomer 1 (ISP1) medium. Endophytic actinomycetes UICC B-92 could be grown faster on Gellan Gum added ISP2 medium than wihout Gellan Gum. Gellan Gum is agar substitute gelling agent. It is water soluble anionic polisaccharide that is produced Sphingomonas elodea bacteria. Its component lead to biodegradable characteristics microbiologically and suitable for the growth of microbes. Therefore, we can use Gellan Gum added ISP2 medium for growing and storaging with freeze dry methods.