THE JOURNAL OF TROPICAL LIFE SCIENCE
Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima
of alternate bioactive compounds isolated from microbial endophytes from plants
may lead to better solution against antibiotic resistance. Main objective of
this research is to determine the bioactivity of antibacterial compounds
produced by endophytic actinomycetes, which was isolated from stem bark of Neesia
altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds
was assayed using Thin Layer Chromatography (TLC) by employing bioautography.
Bioactivity was assayed using crude extract against five microorganisms using
agar diffusion methods. Tetracycline was used as a positive control. The result
of antibacterial activity from endophytic actinomycetes isolate revealed that
the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus
cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella
flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive
compounds of endophytic actinomycetes has an antibacterial activity against
cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm.
Each of TLC fraction was able to inhibit growth of B. cereus at a minimum
concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by
endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of
that compounds are narrow spectrum. The bioactive compound isolated from
endophytic actinomycetes may have other implications such as anticancer,
antifungal, and antioxidant.