BIOEKSPERIMEN

0.00 Avg rating0 Votes
Series:Volume 3
Publisher:Universitas Muhammadiyah
Genres:Jurnal Nasional
Authors:
Pages:10 pages
Binding: Hardcover
ISSN:0024601365

POTENSI EKSTRAK ETANOL BATANG KAPUK RANDU SEBAGAI ANTIBAKTERI
The Potency of Ethanolic Extract from Randu Kapok as Antibacterial Agent

Abstract-The aim of this research is to study inhibited ability of antibacterial compound were isolated from stem of Randu. That extracts were tested for their antibacterial activity to Escherichia coli, Staphylococcus aureus, and Bacillus cereus with disc and dilution methods. Phytochemistry were used for knowing bioactive compounds of that extracts. The extract of 30% ethanol were able to suppress E. coli on 10 mg/mL concentration with 3.8 mm zone of inhibition, S. aureus with 3.6 mm zone of inhibition, and B. cereus with 4.3 mm zone of inhibition. Minimum Inhibitory Concentration (MIC) of 30% ethanol on 8 mg/mL concentration for E. coli, 6 mg/mL for S. aureus and B. cereus. The extract of 50% ethanol were able to suppress E. coli on 10 mg/mL concentration with
4.0 mm zone of inhibition, S. aureus with 4.2 mm zone of inhibition, and B. cereus with 3.9 mm zone of inhibition. Minimum Inhibitory Concentration (MIC) of 50% ethanol on 8 mg/mL concentration for E. coli, 10 mg/mL for S. aureus and 8 mg/mL for B. cereus. The bioactive compounds were able to inhibit the growth of test bacteria are saponin, flavonoid and tannin.

Abstrak-Penelitian ini bertujuan mengetahui daya hambat senyawa antibakteri batang kapuk randu terhadap bakteri Escherichia coli, Staphylococcus aureus, dan Bacillus cereus. Pengujian aktivitas antibakteri menggunakan metode difusi cakram, dilusi cair, nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) terhadap ketiga jenis bakteri. Uji fitokimia dilakukan untuk mengetahui senyawa bioaktif ekstrak etanol batang kapuk randu yang dapat menghambat ketiga jenis bakteri patogen. Aktivitas antibakteri tertinggi pada ekstrak etanol 30% dengan diameter zona hambat pada konsentrasi 10 mg/mL sebesar 3,8 mm terhadap E. coli; 3,6 mm terhadap S. aureus; dan 4,3 mm terhadap B. cereus. KHM ekstrak etanol 30% pada konsentrasi 8 mg/mL terhadap E. coli; 6 mg/ mL terhadap S. aureus; dan 6 mg/mL terhadap B. cereus; KBM pada konsentrasi 10 mg/mL terhadap S. aureus. Aktivitas antibakteri tertinggi pada ekstrak etanol 50% dengan diameter zona hambat pada konsentrasi 10 mg/mL sebesar 4 mm terhadap E. coli; 4,2 mm terhadap S. aureus; dan 3,9 mm terhadap
B. cereus. KHM ekstrak etanol 50% pada konsentrasi 8 mg/mL terhadap E. coli; 10 mg/mL terhadap S. aureus; dan 8 mg/mL terhadap B. cereus; KBM pada 10 mg/mL terhadap B. cereus. Senyawa bioaktif yang berperan menghambat pertumbuhan ketiga jenis bakteri ialah saponin, flavonoid dan tanin.

Reviews

Required fields are marked *. Your email address will not be published.